Журнал "Макрогетероциклы"

For the first time, magnesium octa[(4’-benzo-15-crown-5)oxy]phthalocyanine (Mgcr₈Pc) was explored as a potential PDT agent. Presence of crown-containing fragments in the periphery of the tetrapyrrole macrocycle makes the compound soluble in water. Solutions of Mgcr₈Pc in water and in phosphate buffer (PBS) are stable for a long time. Modification of Mgcr₈Pc solutions in PBS with sodium deoxycholate led to partial monomerization of Mgcr₈Pc and hence to formation of a fluorescent species. The cytotoxicity of Mgcr₈Pc was determined with respect to HeLa cells and its accumulation/localization in the cells was studied by fluorescence microscopy. The increase in signal intensity indicates the accumulation of Mgcr₈Pc in HeLa cells over time. The cell morphology was found to remain practically intact when subjected to Mgcr₈Pc at concentration of 5.00 mM for 24 h. The results obtained allow us to continue the study of this interesting class of compounds.