Журнал "Макрогетероциклы"

Anastasiya E. Shchelkunova,^a,b@ Ekaterina V. Boltukhina,^a Valentina D. Rumyantseva,^b,c Igor’ P. Shilov,^c and Salis D. Karakotov^a

^aAO Shchelkovo Agrokhim, 141101 Shchelkovo, Russia

^bMIREA – Russian Technological University, 119571 Moscow, Russia

^cFryazino Branch of the Kotelnikov Institute of Radioengineering and Electronics, Russian Academy of Sciences, 141190 Fryazino, Russia

^@Corresponding author E-mail: nova2305@mail.ru

DOI: 10.6060/mhc190658s

Macroheterocycles 2019 12(4) 382-388

A method for the preparation of 2,4-di(α-methoxyethyl)deuteroporphyrin IX ytterbium complex dipotassium salt from hemin with the use of chromatographic-mass spectrometric analysis of the impurity composition of the intermediate products is proposed. This complex is an efficient fluorophore and can become a base for the creation of diagnostic products for benign and malignant neoplasms as well as pathological skin conditions. An Agilent 1260 Infinity liquid chromatograph equipped with quadrupole mass selective and multiwave spectrophotometric detectors is used for chromatographic separation. The results of the chromatographic-mass spectrometric analysis of the presented compounds are provided for the first time. The substances and their impurities are identified by comparison with the theoretical molecular weights. It is shown that the chromatographic-mass spectrometric analysis of the intermediate technical products in the course of the synthesis makes it possible to significantly decrease the number of purification operations at all the synthesis stages. The spectral properties of the complex are studied on a Shimadzu UV-1800 double-beam spectrophotometer. The 4f luminescence is measured on a laser-fiber fluorimeter for near-infrared luminescence diagnostics (the know-how of the Fryazino Branch of the Kotelnikov Institute of Radioengineering and Electronics, Russian Academy of Sciences, Fryazino, Russia).